BmECM25, from the silkworm Bombyx mori,is an extracellular matrix protein |
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| Affiliation: | 1. Department of Nanoscience and Nanoengineering, Dokuz Eylül University, İzmir, Turkey;2. Department of Chemistry, Dokuz Eylül University, İzmir, Turkey;1. Chemistry Center and Chemistry Department, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal;2. Chemistry Department and CQ-VR, University of Trás-os-Montes e Alto Douro, Apartado 1013, 5001-801, Vila Real Codex, Portugal;3. REQUIMTE, Instituto Superior de Engenharia do Porto, 4200-072, Porto, Portugal;4. Physics Center, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal;5. BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940, Leioa, Spain;6. IKERBASQUE, Basque Foundation for Science, Bilbao, 48013, Spain;3. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;4. University of Chinese Academy of Sciences, Beijing 100049, China;5. Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China;2. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, KS 66160, USA |
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| Abstract: | BmECM25 (previously reported as BmVMP25) was previously predicted as a gene encoding the vitelline membrane protein in silkworm, Bombyx mori. In this study, we investigated the detail temporal and spatial patterns of BmECM25 protein. Western blot results showed that BmECM25 was expressed in the follicular epithelium cells from stages −6 to +1, and was then secreted into the oocytes. However, the abundance of BmECM25 decreased during the subsequent oogenesis and finally disappeared in the mature follicles. Immunofluorescence detection showed that BmECM25 locates inside the VM layer and forms a discontinuous layer. These features of BmECM25 suggest that it is an oocyte membrane matrix protein, not a vitelline membrane protein. |
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| Keywords: | Oogenesis Follicular epithelium cell Vitelline membrane protein |
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