Lipids in plant tissue cultures. I. The fatty acid composition of triglycerides in rape and turnip rape cultures

Rape (Brassica napus cv. Target) and turnip rape (Brassica campestris cv. Echo) were grown as callus cultures. The lipids isolated from these cultures were found to contain substantial amounts of monogalactosyl diglycerides, digalactosyl diglycerides, and phosphatidyl glycerol, whereas sulfolipids, ethanolamine phosphoglycerides and choline phosphoglycerides occurred in small proportions. The constituent fatty acids of the triglycerides from the two tissue cultures contained more than 50% of 18:3, but they were free of 22:1, which is a major fatty acid in the triglycerides from seed oils of both rape and turnip rape.