Determination of plasma trace elements in tumor-bearing animals by proton-induced X-ray emission spectroscopy |
| |
Authors: | M Fogle B Daly M Evans E L B Justiniano C J Kovacs J L Shinpaugh L H Toburen |
| |
Institution: | (1) Department of Physics, East Carolina University, USA;(2) Division of Radiation Biology and Oncology, Department of Radiation Oncology, East Carolina University School of Medicine, Greenville, NC |
| |
Abstract: | Although altered levels of circulating essential trace elements are known to accompany malignant disease, the lack of sensitivity
of conventional detection methods has generally limited their study to clinical conditions involving extensive disease (i.e.,
significant tumor burden). As such, the application of altered trace element levels as potential prognostic guides or as response
indicators subsequent to treatment has been of limited use. During this study, proton-induced X-ray emission spectroscopy
was evaluated as a tool to determine trace element imbalances in a murine tumor model. Using plasma from C57B1/6 mice bearing
the syngeneic Lewis lung carcinoma (LLCa), levels of Fe, Cu, and Zn, as well as changes in the Cu/Zn ratio, were measured
in animals carrying an increasing primary tumor burden. The plasma levels of Fe, Cu, and Zn were found to decrease significantly
7 d following implants of LLCa cells with no significant change observed in the Cu/Zn ratio. By d 21, however, an increase
in the Cu/Zn ratio was found to accompany increased growth of the LLCa tumor; the plasma levels of Cu had returned to normal
levels, whereas both the Fe and Zn plasma levels remained lowered. Collectively, the results suggest that although a net change
in individual plasma trace element concentrations might not be accurately associated with tumor growth, a clear relationship
was established between the Cu/Zn ratio and tumor size. |
| |
Keywords: | Neoplasm iron copper zinc Lewis lung carcinoma proton-induced x-ray emission trace element blood plasma |
本文献已被 SpringerLink 等数据库收录! |
|