Effect of Combined Changes in Culture Medium and Incubation Conditions on the Regeneration from Immature Embryos of Elite Varieties of Winter Wheat |
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| Authors: | Tamás Cecília Szűcs Péter Rakszegi Mariann Tamás László Bedő Zoltán |
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| Affiliation: | (1) Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462 Martonvásár, POB 19, Hungary;(2) Department of Plant Physiology, Eötvös Loránd University, H-1518 Budapest Pf. 32, Hungary |
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| Abstract: | In this study, tissue culture method for plant regeneration from immature embryos of elite Hungarian winter wheat varieties was established. The influence of the growth regulators and the concentration of macroelements in the regeneration medium and of the incubation temperature and light intensity on regeneration frequency were investigated. The most noticeable effect on regeneration frequency was achieved by simultaneously reducing both the incubation temperature to 23 °C and the concentration of macroelements in the regeneration medium to half-strength. This modification increased the average regeneration frequency from about 10–78%. Changes in the light intensity and temperature gave an average plant regeneration frequency of 83%. |
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| Keywords: | immature embryo in vitro culture light shoot regeneration temperature Triticum aestivum L. |
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