Effect of adenine nucleotide moiety of coenzyme A on phosphotransacetylase

Structural analogues of CoA lacking in aletheine moiety as well as in pantetheine moiety were found to compete with CoA in the phosphotransacetylase reaction. Moreover, the latter compound has induced an ability of the enzyme to acetylate pantetheine in the absence of CoA, which was inhibited by desulfo-CoA. These results suggest that adenine nucleotide moiety of CoA plays an essential role in being recognized as CoA by phosphotransacetylase.