Alkylation-induced genotoxicity as a predictor of DNA repair deficiency following experimental oral carcinogenesis |
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| Authors: | Juliana G Carvalho Juliana Noguti Victor Hugo P da Silva Rogério A Dedivitis Marcello Franco Daniel A Ribeiro |
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| Institution: | (1) Department of Biosciences, Federal University of S?o Paulo UNIFESP, Av. Ana Costa, 95, Vila Mathias, Santos, SP, Brazil;(2) Department of Pathology, Federal University of S?o Paulo, UNIFESP, S?o Paulo, SP, Brazil;(3) Department of Head and Neck Surgery, Ana Costa Hospital and Santa Casa Santos, Santos, SP, Brazil; |
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| Abstract: | The aim of this study was to evaluate alkylation induced genotoxicity as a result of DNA repair deficiency during 4-nitroquinoline
1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) assay. Male Wistar rats were distributed
into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks.
Ten animals were used as negative control. Blood samples and oral mucosa cells collected from all animals were divided into
two aliquots of 20 μL each to study basal DNA damage and DNA damage due to genotoxin sensitivity. The first aliquot was processed
immediately for comet assay to assess basal DNA damage. The second aliquot was treated with a known genotoxin, methylmetanesulfonate.
Significantly greater DNA damage was noticed to oral mucosa cells from 4, and 12 weeks post-treatment. Peripheral blood cells
did show statistically significant differences (P < 0.05) after 20 weeks-group (squamous cell carcinoma). In conclusion, alkylation induced genotoxicity as a result of DNA
repair deficiency is present in oral mucosa cells following oral experimental carcinogenesis. |
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