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RNA synthesis in the presence of transfer RNa by DNA-dependent RNA polymerase II from mouse ascites sarcoma cells |
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| Authors: | H Misumi H Hidaka T Shohmori T Oda |
| Institution: | 1. Department of Clinical Neurochemistry, Institute for Neurobiology, 2-5-1, Shikata-cho, Okayama 700, Japan;2. Department of Biochemistry, Cancer Institute, Okayama University Medical School, 2-5-1, Shikata-cho, Okayama 700, Japan |
| Abstract: | RNA polymerase II from mouse sarcoma cells catalyzed the incorporation of UMP into an acid-insoluble fraction in the presence of tRNA. This reaction was not affected by DNase or actinomycin D but was inhibited by α-amanitin. This reaction was dependent on nucleoside triphosphate and manganese ions. RNA synthesized in the presence of tRNA could be digested with RNase A. These results suggest that the RNA synthesis by RNA polymerase II from mouse sarcoma is dependent on the presence of tRNA. |
| Keywords: | To whom correspondence should be addressed |
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