Molecular characterization of novel low-molecular-weight glutenin genes inAegilops longissima |
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| Authors: | Z Huang H Long Q T Jiang Y M Wei Z H Yan Y-L Zheng |
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| Institution: | (1) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830, China;(2) Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, K1A 0C6, ON, Canada;(3) Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, 31905, Israel; |
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| Abstract: | Extensive genetic variations of low-molecular-weight glutenin subunits (LMW-GS) and their coding genes were found in the wild
diploid A- and D-genome donors of common wheat. In this study, we reported the isolation and characterization of 8 novel LMW-GS
genes fromAe.longissima Schweinf. & Muschl., a species of the sectionSitopsis of the genusAegilops, which is closely related to the B genome of common wheat. Based on the N-terminal domain sequences, the 8 genes were divided
into 3 groups. A consensus alignment of the extremely conserved domains with known gene groups and the subsequent cluster
analysis showed that 2 out of the 3 groups of LMW-GS genes were closely related to those from the B genome, and the remaining
was related to those from A and D genomes of wheat andAe. tauschii. Using 3 sets of gene-group-specific primers, PCRs in diploid, tetraploid and hexaploid wheats andAe. tauschii failed to obtain the expected products, indicating that the 3 groups of LMW-GS genes obtained in this study were new members
of LMW-GS multi-gene families. These results suggested that theSitopsis species of the genusAegilops with novel gene variations could be used as valuable gene resources of LMW-GS. The 3 sets of group-specific primers could
be utilized as molecular markers to investigate the introgression of novel alien LMW-GS genes fromAe. longissima into wheat. |
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