Strain-dependent expression of metabolic proteins in the mouse hippocampus |
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Authors: | Daniela D Pollak Narkhyun Bae Gehan Mostafa Harald Hoeger |
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Institution: | (1) Department of Physiology, Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria;(2) Division of Pediatric Neuroscience, Department of Pediatrics, Medical University of Vienna, Vienna, Austria;(3) Division of Laboratory Animal Science and Genetics, Core Unit of Biomedical Research, Medical University of Vienna, Vienna, Austria |
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Abstract: | Individual mouse strains differ significantly in terms of behavior and cognitive function. Strain-specific variation of metabolic
protein levels in the hippocampus among various commonly used mouse strains, however, has not been investigated yet. A proteomic
approach based on two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry high capacity ion trap (HCT)]
has been chosen to address this question by determining strain-dependent levels of metabolic proteins in hippocampal tissue
of four inbred and one outbred mouse strain. Statistical analysis of protein spots on 2-DE gels of the individual strains
(n = 10) revealed significant strain-dependent differences in densities of 39 spots. Subsequent HCT analysis led to the identification
of 22 different metabolic proteins presenting with differential protein levels among the five mouse strains investigated.
Among those are proteins concerned with the metabolism of amino acid, nucleic acid, carbohydrate and energy. Moreover, proteins
known to play a pivotal role in the processes of learning and memory, such as calcium/calmodulin-dependent protein kinase type II alpha chain, were found to present with significant inter-strain variability, which is also in agreement with our previous reports. Strain-specific
protein levels of metabolic proteins in the mouse hippocampus may provide some insight into the molecular underpinnings and
genetic determination of strain-dependent neuronal function. Furthermore, data presented herein emphasize the significance
of the genetic background for the analysis of metabolic pathways in the hippocampus in wild-type mice as well as in gene-targeting
experiments. |
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