Fluorescence changes accompanying short-term light adaptations in photosystem I and photosystem II of the cyanobacterium <Emphasis Type="Italic">Synechocystis</Emphasis> sp. PCC 6803 and phycobiliprotein-impaired mutants: State 1/State 2 transitions and carotenoid-induced quenching of phycobilisomes |
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Authors: | Igor N Stadnichuk Evgeny P Lukashev Irina V Elanskaya |
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Institution: | (1) A.N. Bakh Institute of Biochemistry Russian Academy of Sciences, Leninski Prospekt 33, Moscow, Russia;(2) Biological Department, Moscow Lomonosov State University, Mendeleeva Street 2, Moscow, Russia |
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Abstract: | The features of the two types of short-term light-adaptations of photosynthetic apparatus, State 1/State 2 transitions, and
non-photochemical fluorescence quenching of phycobilisomes (PBS) by orange carotene-protein (OCP) were compared in the cyanobacterium
Synechocystis sp. PCC 6803 wild type, CK pigment mutant lacking phycocyanin, and PAL mutant totally devoid of phycobiliproteins. The permanent
presence of PBS-specific peaks in the in situ action spectra of photosystem I (PSI) and photosystem II (PSII), as well as
in the 77 K fluorescence excitation spectra for chlorophyll emission at 690 nm (PSII) and 725 nm (PSI) showed that PBS are
constitutive antenna complexes of both photosystems. The mutant strains compensated the lack of phycobiliproteins by higher
PSII content and by intensification of photosynthetic linear electron transfer. The detectable changes of energy migration
from PBS to the PSI and PSII in the Synechocystis wild type and the CK mutant in State 1 and State 2 according to the fluorescence excitation spectra measurements were not
registered. The constant level of fluorescence emission of PSI during State 1/State 2 transitions and simultaneous increase
of chlorophyll fluorescence emission of PSII in State 1 in Synechocystis PAL mutant allowed to propose that spillover is an unlikely mechanism of state transitions. Blue–green light absorbed by
OCP diminished the rout of energy from PBS to PSI while energy migration from PBS to PSII was less influenced. Therefore,
the main role of OCP-induced quenching of PBS is the limitation of PSI activity and cyclic electron transport under relatively
high light conditions. |
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Keywords: | Cyanobacterium Fluorescence emission Fluorescence excitation Photosynthetic action spectra Photosystem I Photosystem II Phycobilisome(s) State transitions |
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