| Differences in nutrient uptake between the fat body and embryonic primary cultures of silkworm （Bombyx mori） |
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| 作者姓名： | LEILA MATINDOOST JALAL J. SENDI HOORIEH SOLEIMAN JAHI KAYVAN ETEBARI |
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| 作者单位： | [1]Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht [2]Department of Virology, School of Medical Science, Tarbiat Modarres, Tehran [3]Department of Sericulture, Faculty of Natural Resources, University of Guilan, Somehesara, Iran |
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| 摘 要： | Nutrition utilization and by-product formation in cultured insect cells has been investigated in several insect cells and has been of great interest to cell culturists and physiologists. In this research the biochemical changes in embryonic and fat body primary cultures of silkworm, Bombyx mori, have been compared. TC-100 medium supplemented with 10% and 20% FBS was used in embryonic and fat body primary cultures, respectively. Medium was renewed every week and the amount of glucose, uric acid, urea, total protein and alkaline phosphatase were measured in the samples from medium of primary cultures using spectrophotometeric methods. All biochemical macromolecules except uric acid showed significant changes. Glucose decreased in embryonic tissues, while in fat body culture its amount increased. Urea accumulation in embryonic culture was higher than in the fat body cultures. Since urea is a by-product, this accumulation could be due to higher utilization of amino acids. Total protein showed considerable changes and was consumed by embryonic culture more than the fat body＇ s. Alkaline phosphatase showed stronger activity in embryonic cells.
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| 关 键 词： | 肥胖 营养摄取 胚胎 蚕学 |
| 收稿时间： | 2005-09-20 |
| 修稿时间： | 2005-09-20 |
Differences in nutrient uptake between the fat body and embryonic primary cultures of silkworm (Bombyx mori) |
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| Authors: | LEILA MATINDOOST JALAL J SENDI HOORIEH SOLEIMAN JAHI KAYVAN ETEBARI |
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| Institution: | Department of Plant Protection, Faculty of Agriculture, University of Guilan, Rasht;. Department of Virology, School of Medical Science, Tarbiat Modarres, Tehran;. Department of Sericulture, Faculty of Natural Resources, University of Guilan, Somehesara, Iran |
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| Abstract: | Abstract Nutrition utilization and by-product formation in cultured insect cells has been investigated in several insect cells and has been of great interest to cell culturists and physiologists. In this research the biochemical changes in embryonic and fat body primary cultures of silkworm, Bombyx mori , have been compared. TC-100 medium supplemented with 10% and 20% FBS was used in embryonic and fat body primary cultures, respectively. Medium was renewed every week and the amount of glucose, uric acid, urea, total protein and alkaline phosphatase were measured in the samples from medium of primary cultures using spectrophotometeric methods. All biochemical macromolecules except uric acid showed significant changes. Glucose decreased in embryonic tissues, while in fat body culture its amount increased. Urea accumulation in embryonic culture was higher than in the fat body cultures. Since urea is a by-product, this accumulation could be due to higher utilization of amino acids. Total protein showed considerable changes and was consumed by embryonic culture more than the fat body's. Alkaline phosphatase showed stronger activity in embryonic cells. |
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| Keywords: | biochemical Bombyx mori embryo fat body insect cell primary culture silkworm |
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