Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268 |
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| Authors: | B Boizet D Villeval P Slos M Novel G Novel A Mercenier |
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| Institution: | Institut de Recherche de Biologie Appliquée, Université de Caen, France. |
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| Abstract: | A 4.4-kb XhoI fragment of Streptococcus lactis L13 (Z268) lactose plasmid pUCL13, containing the beta-D-phosphogalactoside galactohydrolase (P-beta Gal; EC 3.2.1.85)-coding gene has been cloned in Escherichia coli. Further subcloning and deletion of this fragment allowed localization of the P-beta Gal-coding gene (pbg) on a minimal 1.8-kb segment. Expression of P-beta Gal activity was constitutive and was not regulated by glucose in E. coli. The presence of P-beta Gal activity was correlated with the production of a 56.5-kDa protein in E. coli minicells. The nucleotide sequence of the cloned gene was determined and potential promoter structural elements were identified. |
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