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Cell wall carbohydrates from fruit pulp of Argania spinosa: structural analysis of pectin and xyloglucan polysaccharides
Authors:Aboughe-Angone Sophie  Nguema-Ona Eric  Ghosh Partha  Lerouge Patrice  Ishii Tadashi  Ray Bimalendu  Driouich Azeddine
Affiliation:UMR CNRS 6037, IFRMP23, Université de Rouen, Mont Saint Aignan 76821, France. sophie.abougheangone@etu.univ-rouen.fr
Abstract:Isolated cell walls of Argania spinosa fruit pulp were fractionated into their polysaccharide constituents and the resulting fractions were analysed for monosaccharide composition and chemical structure. The data reveal the presence of homogalacturonan, rhamnogalacturonan-I (RG-I) and rhamnogalacturonan-II (RG-II) in the pectic fraction. RG-I is abundant and contains high amounts of Ara and Gal, indicative of an important branching in this polysaccharide. RG-II is less abundant than RG-I and exists as a dimer. Structural characterisation of xyloglucan using enzymatic hydrolysis, gas chromatography, MALDI-TOF-MS and methylation analysis shows that XXGG, XXXG, XXLG and XLLG are the major subunit oligosaccharides in the ratio of 0.6:1:1.2:1.6. This finding demonstrates that the major neutral hemicellulosic polysaccharide is a galacto-xyloglucan. In addition, Argania fruit xyloglucan has no XUFG, a novel xyloglucan motif recently discovered in Argania leaf cell walls. Finally, the isolation and analysis of arabinogalactan-proteins showed that Argania fruit pulp is rich in these proteoglycans.
Keywords:Argania spinosa   Cell wall   Fruit pulp   MALDI-TOF-MS   Pectin   Xyloglucan
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