CFD simulation coupled with population balance equations for aerated stirred bioreactors |
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Authors: | Hu Zhang Kai Zhang Shengdi Fan |
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Institution: | 1. Advanced Center for Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, United Kingdom;2. Department of Chemical Engineering, The University of Adelaide, SA 5005, Australia;3. National Engineering Lab for Biomass Power Generation Equipment, North China Electric Power Beijing 102249 P. R. China;4. College of Life Science, Life Science College, Dalian Nationality University, Dalian, 116600, P. R. China |
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Abstract: | Mammalian cells have been widely used to produce therapeutic proteins in stirred bioreactors in suspension culture. Local hydrodynamics can have a great impact on cell proliferation and protein synthesis, but there are few reports on spatial heterogeneity of nutrients, gas bubbles, and mass transfer coefficients. We have employed computational fluid dynamics (CFD) coupled with population balance equations to study local hydrodynamics in a 20 L stirred bioreactor. The flow patterns, energy dissipation rates, gas volume fraction, gas bubble size distribution and local mass transfer coefficient have been displayed throughout the whole bioreactor. Their implications for mammalian cell culture have been discussed. This study provides an insight into rational design and optimum operation conditions in a stirred bioreactor for mammalian cell cultivation. |
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Keywords: | CFD Gas‐liquid flow Mammalian cell culture Stirred bioreactor |
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