Tracing the origins of stocks of the endangered species <Emphasis Type="Italic">Primula sieboldii</Emphasis> using nuclear microsatellites and chloroplast DNA |
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Authors: | M Honjo S Ueno Y Tsumura T Handa I Washitani R Ohsawa |
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Institution: | (1) Graduate School of Agricultural and Life Science, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;(2) Department of Forest Genetics, Forestry and Forest Products Research Institute, Tsukuba Ibaraki, 305-8687, Japan;(3) Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Ibaraki, 305-8572, Japan |
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Abstract: | We examined the origins of cultivated stocks of the endangered species Primula sieboldii at the individual plant level by using an assignment test based on eight microsatellite loci and regional features of chloroplast
DNA (cpDNA) variation of wild populations. To confirm that we had sufficient information for estimating the origins of the
stocks, we performed an assignment test with 920 genets that we collected from 32 wild populations with known origins. The
test assigned 99.6% of the genets to the population from which they had been sampled, confirming the suitability of the method.
We then performed the assignment test with 29 cultivated stocks. The alleged origins of 19 were confirmed by microsatellite
and cpDNA variations. In contrast, the alleged origins of five were rejected by both markers. Five stocks, which do not have
a reference population located within 30 km of their reputed origin, were not assigned to any population. Stocks whose alleged
origins were rejected are inappropriate as restoration materials, because their introduction might disturb local gene pools.
Six stock haplotypes could not be detected in wild populations. This may suggest the loss of genetic diversity in the wild
and the value of stocks as a gene bank. The genetic method used in this study will also be helpful to detect cryptic invasion
by nonendemic genotypes or to trace the origins of plants collected for commercial purposes, a threat to many endangered species. |
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Keywords: | Assignment test Ex situ stocks Gene bank Genetic restoration Traceability |
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