Conversion of 20alpha-hydroxy-4-pregnen-3-one to 20alpha-hydroxy-5alpha-pregnan-3-one and 5alpha-pregnane-3alpha, 20alpha-diol by rat anterior pituitary |
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Authors: | F V Nowak H J Karavolas |
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Institution: | Department of Physiological Chemistry The Endocrinology-Reproductive Physiology Program and The Waisman Center on Mental Development The University of Wisconsin Madison, Wisconsin 53706 U.S.A. |
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Abstract: | 3H-20α-hydroxy-4-pregnen-3-one was incubated with anterior pituitaries from proestrous rats. The metabolic products, identified by reverse isotopic dilution and purification to constant specific activity, were 20α-hydroxy-5α-pregnan-3-one (23.0%) and 5α-pregnane-3α,20α-diol (11.4%). These are qualitatively the same metabolites which result from incubation of 20α-hydroxy-4-pregnen-3-one with medial basal hypothalamus. 68.8% of the recovered radioactivity remained as 20α-hydroxy-4-pregnen-3-one. These three compounds accounted for all of the recovered radioactivity. |
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