Lactogenic response of mouse mammary explants from different days of pregnancy to placental lactogen and pituitary prolactin |
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Authors: | Judy Ways Edith Markoff Linda Orgen Frank Talamantes |
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Affiliation: | (1) Division of Natural Sciences and Oakes College, University of California, 95064 Santa Cruz, California;(2) Thimann Laboratories, University of California, 95064 Santa Cruz, California |
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Abstract: | Summary The lactogenic response of mouse mammary gland explants to human placental lactogen (hPL) and ovine pituitary prolactin (oPRL) was examined on days 10 to 18 of pregnancy by measuring3H-amino acid incorporation into calcium-rennin precipitable casein. To determine the lactogenic response of the explants, the mean slopes of dose-response curves were calculated for each hormone treatment. Slope means of dose-response curves for oPRL and hPL did not differ from each other on any day of pregnancy examined. A triphasic pattern of response was suggested when slope means of dose-response curves for both hormones were plotted as a function of day of gestation. Peak responses were observed on days 10, 13 and 17–18. Combinations of oPRL and hPL, in ratios of oPRL:hPL=2∶1 and oPRL:hPL=1∶2, also produced a triphasic pattern of sensitivity very similar to that produced by either hormone alone. These results suggest that mouse mammary explants may be more sensitive to oPRL and hPL on days 10, 13 and 17–18 of pregnancy. This work was supported by grants from the National Science Foundation (76-01928) and the National Institutes of Health (5 S06RR08132-0251) to F. Talamantes. |
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Keywords: | mouse pregnancy human placental lactogen ovine pituitary prolactin mammary gland casein synthesis |
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