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AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7 |
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| Authors: | Abdel-Ghany Salah E Burkhead Jason L Gogolin Kathryn A Andrés-Colás Nuria Bodecker Jared R Puig Sergi Peñarrubia Lola Pilon Marinus |
| Institution: | a Department of Biology, Colorado State University, Anatomy/Zoology Building, Fort Collins, CO 80523, USA b Dpto. Bioquímica y Biología Molecular, Facultad Ciencias Biológicas, Universidad de Valencia, C/ Dr. Moliner 50, 46100 Burjassot, Valencia, Spain |
| Abstract: | In plant chloroplasts two superoxide dismutase (SOD) activities occur, FeSOD and Cu/ZnSOD, with reciprocal regulation in response to copper availability. This system presents a unique model to study the regulation of metal-cofactor delivery to an organelle. The Arabidopsis thaliana gene AtCCS encodes a functional homolog to yeast Ccs1p/Lys7p, a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. AtCCS mRNA expression levels are upregulated in response to Cu-feeding and senescence. We propose that AtCCS expression is regulated to allow the most optimal use of Cu for photosynthesis. |
| Keywords: | CCS copper chaperone for Cu/Zn superoxide dismutase Cu/ZnSOD Cu/Zn superoxide dismutase FeSOD Fe superoxide dismutase SOD superoxide dismutase GFP green fluorescent protein CaMV35S cauliflower mosaic virus 35S promoter |
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