QPCR: Application for real-time PCR data management and analysis |
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| Authors: | Stephan Pabinger Gerhard G Thallinger René Snajder Heiko Eichhorn Robert Rader and Zlatko Trajanoski |
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| Institution: | (1) Institute for Genomics and Bioinformatics, Graz University of Technology, Petersgasse 14, 8010 Graz, Austria;(2) Christian Doppler Laboratory for Genomics and Bioinformatics, , Petersgasse 14, 8010 Graz, Austria;(3) Development Anti-Infectives Microbiology, Sandoz GmbH, Biochemiestrasse 10, 6250 Kundl, Austria |
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| Abstract: | Background Since its introduction quantitative real-time polymerase chain reaction (qPCR) has become the standard method for quantification
of gene expression. Its high sensitivity, large dynamic range, and accuracy led to the development of numerous applications
with an increasing number of samples to be analyzed. Data analysis consists of a number of steps, which have to be carried
out in several different applications. Currently, no single tool is available which incorporates storage, management, and
multiple methods covering the complete analysis pipeline. |
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