The dissociation and separation of bovine adenohypophysial cells |
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Authors: | Michael A Hirsch Harry Lipner Robert C Leif |
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Institution: | (1) Department of Biological Sciences, Florida State University, 32306 Tallahassee, Florida;(2) Papanicolaou Cancer Research Institute, 33136 Miami, Florida |
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Abstract: | Bovine adenohypophysial tissue was dissociated by sequential enzymatic incubation in a continuous flow system. Dispersed cells
separated into discrete fractions after centrifugation in isopycnic bovine serum albumin gradients. The dispersed and separated
cells were prepared for microscopic identification and differential counts by centrifugal cytology. Radioimmunoassays for
LH, FSH, TSH, and Prl were used to corroborate the differential counts and determine the homogeneity of the fractions. The
thyrotrophs banded at an average density (
) of 1.0417, the FSH-secretory cells at
, the LH-secretory cells at
, and the Prl-secretory cells at
. A 7–16 fold enrichment of different cell populations was possible. In bovine hypophyses each hormone appears to be formed
by specific cells: the average TSH concentrations of the thyrotrophs were 5.1 pg/cell and the average LH and FSH concentrations
were 4.7 and 4.9 pg/cell for LH-and FSH-secreting cells, respectively. The average Prl concentration was 4.9 pg/cell for Prl-secreting
cells. |
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Keywords: | Dissociation bovine adenohypophysial cells separation adenohypophysial cells bovine adenohypophysial cells adenohypophysial cells bovine isopycnic bovine serum albumin gradient gradient isopycnic bovine serum albumin centrifugal cytology pituitary |
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