Affiliation: | (1) Molecular Genetics of Industrial Microorganisms, Wageningen University, Dreijenlaan 2, , 6703 Wageningen, HA , The Netherlands;(2) Microbiology, Utrecht University, Padualaan 8, 3584 Utrecht, CH , The Netherlands;(3) Mycology Group, Technical University of Denmark, Soltofts Plads, 2800 Lyngby, DK, Denmark;(4) Centraalbureau voor Schimmelcultures, P.O. Box 85167, 3508 Utrecht, AD , The Netherlands;(5) Laboratory of Phytopathology, Wageningen University, P.O. Box 8025, 6700 Wageningen, EE , The Netherlands;(6) CatchMabs, P.O. Box 134, 6700 Wageningen, AC, The Netherlands;(7) Fungal Genetics and Technology Consultancy, P.O. Box 396, 6700 Wageningen, AJ, The Netherlands |
Abstract: | A strain from the group of black Aspergilli was analysed in detail to determine the species to which it belongs. A detailed analysis of morphology, RFLP patterns and metabolite profiles was carried out. In addition, a phylogenetic tree was constructed for the black Aspergilli using the ITS and the -tubulin sequences of the individual strains. The new species differs by its poor growth on glycerol and galacturonate and its unique extrolite profile consisting of aurasperone B, nigragillin, asperazine and kotanins. RFLP analysis using three genes as probes also resulted in a unique pattern. These data indicate that the strain was closely related but not identical to Aspergillus foetidus, Aspergillus niger and Aspergillus tubingensis. It was therefore designated as a novel species and named Aspergillus vadensis. |