In vitro synthesis of peroxisomal membrane polypeptides |
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| Authors: | A K?ster M Heisig P C Heinrich W W Just |
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| Affiliation: | 1. Biomedical Ethics Research Program, Mayo Clinic, Rochester, MN, USA;2. Graduate School of Biomedical Sciences, Mayo Clinic, Jacksonville, FL, USA;3. Department of Obstetrics and Gynecology, Mayo Clinic, Rochester, MN, USA;4. Division of Health Care Delivery Research, Robert D. & Patricia E. Kern Center for the Science of Health Care Delivery, Mayo Clinic, Rochester, MN, USA;5. Division of Reproductive Endocrinology and Infertility, Mayo Clinic, Rochester, MN, USA |
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| Abstract: | Peroxisomal membranes containing predominantly integral peroxisome membrane polypeptides were obtained from a highly purified peroxisomal fraction. Following sodium dodecylsulfate polyacrylamide gel electrophoresis three polypeptides with apparent molecular weights of 69, 36, and 22 kDa were isolated and used to raise antibodies in rabbits. Cell-free synthesis of these polypeptides was carried out in an in vitro translational system derived from rabbit reticulocytes. By subjecting peroxisomal membranes to reductive methylation [14C]-radiolabeled mature membrane polypeptides were obtained. The comparison of the three mature integral peroxisome membrane polypeptides with their corresponding in vitro synthesis products revealed no size differences indicating the lack of recognizable presequences for these peroxisomal membrane polypeptides. |
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