Functional domains in introns: trans-acting and cis-acting regions of intron 4 of the cob gene |
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Authors: | P Q Anziano D K Hanson H R Mahler P S Perlman |
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Institution: | 1. Molecular, Cellular and Developmental Biology Program The Ohio State University Columbus, Ohio 43210 USA;2. Chemistry Department and Program in Molecular, Cellular and Developmental Biology Indiana University Bloomington, Indiana 47405 USA;3. Genetics Department The Ohio State University Columbus, Ohio 43210 USA |
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Abstract: | We have sequenced the mutational changes in eight mutants in the open reading frame of intron 4 of the cob gene on yeast mitochondrial DNA. Three have a cis-acting splicing defect, while the other inactivate a trans-recessive intron domain that specifies a trans-acting splicing factor. From phenotypic evidence, including analyses of the allele-specific extra proteins, we have identified a protein (P27) encoded wholly within the intron that appears to be the intron 4 splicing factor (maturase). The evidence suggests that P27 is a secondary translation product resulting from the proteolytic cleavage of a larger precursor encoded by exon and intron sequences, but an alternative model, in which P27 is a primary translation product, has not been ruled out. |
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