Peptides derived from pyruvate dehydrogenase as substrates for pyruvate dehydrogenase kinase and phosphatase. |
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Authors: | P F Davis F H Pettit L J Reed |
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Affiliation: | Clayton Foundation Biochemical Institute and the Department of Chemistry The University of Texas at Austin, Austin, Texas 78712 USA |
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Abstract: | Evidence is presented that phosphopeptides produced by tryptic digestion of phosphorylated pyruvate dehydrogenase are effective substrates for pyruvate dehydrogenase phosphatase and that the dephosphopeptides can serve as substrates for pyruvate dehydrogenase kinase. These findings indicate that the phosphatase and the kinase do not require an intact tertiary structure in pyruvate dehydrogenase, but apparently recognize components of the local primary sequence around the phosphorylation sites. |
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Keywords: | active nonphosphorylated form of pyruvate dehydrogenase inactive phosphorylated form |
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