Application of FAME (fatty acid methyl ester) analysis in the numerical taxonomic determination of bacterial guild structure |
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Authors: | Lawrence M Mallory Gary S Sayler |
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Institution: | (1) Department of Microbiology and the Graduate Program in Ecology, The University of Tennessee, 37996 Knoxville, Tennessee, USA;(2) Present address: Department of Biology, Memphis State University, Memphis, TN, USA |
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Abstract: | Comparative numerical taxonomic analyses, using fatty acid methyl ester (FAME) profiles and phenetic characteristics, were conducted to examine bacterial guild structure in freshwater sediments. Both approaches were used to examine a subset of 60 OTUs obtained from a previously well characterized microbial community in sediments of a shallow fast flowing stream. For both classifications, greater than 80% of the OTUs were recovered in 11 and 12 major groups for FAME and phenetics approaches. However, there was not complete correspondence for the groupings of the 2 classifications, with most FAME groups being distributed among the phenetic groups and 2 phenetic groups not providing usable characteristics for FAME analysis. The results did demonstrate significant taxonomic variation in bacteria capable of occupying the same or a similar fundamental niche. |
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