| 酵母全细胞苯丙氨酸鲜氨酶（PAL）活性的紫外分光光度测定法 |
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| 引用本文： | 杨顺楷 李果龙. 酵母全细胞苯丙氨酸鲜氨酶（PAL）活性的紫外分光光度测定法[J]. 天然产物研究与开发, 1990, 2(1): 59-62 |
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| 作者姓名： | 杨顺楷 李果龙 |
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| 作者单位： | 中国科学院成都生物研究所 成都610015(杨顺楷，赵健身)，中国科学院成都生物研究所86级 硕士生(李果龙) |
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| 摘 要： | 本文报告以L-苯丙氨酸 (L-phe) 为底物,酵母全细胞作酶源,酶促生成产物反式-肉桂酸 (t-Ca)测定苯丙氨解氨酸 (PAP,EC_(4.3.1.5) 活性的紫外分光光度法。测定程序包括标准物质t-Ca的加样试验,绝对回收率试验,线性回归分析的整套定量分析研宄步骤,建立了一套经过修改的Kalghatgi和Subba Rao(1975) PAL 活性测定法。此法具有良好的准确度和精密度,已经用于评价具有PAL活性的酵母菌株在液体培养物中细胞生长和PAL活性形成的时间过程研究。
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| 关 键 词： | PAL活性 紫外分光光度 酵母全细胞 |
DETERMINATION OF PHENYLALANINE AMMONIA-LYASE ACTIVITY OF WHOLE YEAST CELLS BY ULTRAVIOLET SPECTROPHOTOMETRY |
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| Yang Shunkai,Li Guolong,Zhao Jianshen. DETERMINATION OF PHENYLALANINE AMMONIA-LYASE ACTIVITY OF WHOLE YEAST CELLS BY ULTRAVIOLET SPECTROPHOTOMETRY[J]. Natural Product Research and Development, 1990, 2(1): 59-62 |
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| Authors: | Yang Shunkai Li Guolong Zhao Jianshen |
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| Abstract: | A assay procedure of Phenylalanine Ammonia-lyase (PAL, EC4.3.1.5) of whole yeast cells, has been developed for the determination of L-phenylalanine and its deaminonative product trans-cinnamic acid (t-Ca) in the yeast cell culture by an ultroviolet spectrophotometric method. These quantitave procedure included spiked samples of standard t-Ca, absolute recovery test, and linear regression analysis. The method of PAL activity analysis was made by the modificated method described by Kalghatgi and Subba Rao (1975). The assay procedure was accurate and precise. The proposed assay has been applied to evaluate PAL activity of yeast strains and study the time course of cell groth and PAL formation in an aqueous medium. |
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| Keywords: | Phenylalanine Ammonia-lyase activity ultroviolet spe-cpectrophotometric method whole yeast cells |
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