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End labeling of enzymatically decapped mRNA. |
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| Authors: | A Efstratiadis J N Vournakis H Donis-Keller G Chaconas D K Dougall and F C Kafatos |
| Abstract: | A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase. |
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