| 萤火虫萤光素酶基因构建BIV－LTR启动子表达研究体系 |
| |
| 引用本文： | 刘国文,纪永刚,梁臣,刘淑红,陈启民,耿运琪.萤火虫萤光素酶基因构建BIV－LTR启动子表达研究体系[J].病毒学报,1994(4). |
| |
| 作者姓名： | 刘国文 纪永刚 梁臣 刘淑红 陈启民 耿运琪 |
| |
| 作者单位： | 南开大学生命科学学院，中国科学院微生物学研究所 |
| |
| 摘 要： | 萤火虫萤光素酶基因构建ＢＩＶ－ＬＴＲ启动子表达研究体系刘国文，纪永刚，梁臣，刘淑红，陈启民，耿运琪（南开大学生命科学学院天津３０００７１）关键词萤光虫萤光素酶基因（ｌｕｃ），ＢＩＶ－ＬＴＲ，ＢＩＶ－ｔａｔ目前使用最广泛的报道基因是细菌的氯霉素乙酰转移...
|
CONSTRUCTION OF BIV -LTR PROMOTER EXPRESSION SYSTEM WITH FIREFLY LUCIFERASE GENE |
| |
| Liu Guowen JiYonggang Liang Chen Liu Shuhong Chen Qimin Geng Yunqi.CONSTRUCTION OF BIV -LTR PROMOTER EXPRESSION SYSTEM WITH FIREFLY LUCIFERASE GENE[J].Chinese Journal of Virology,1994(4). |
| |
| Authors: | Liu Guowen JiYonggang Liang Chen Liu Shuhong Chen Qimin Geng Yunqi |
| |
| Abstract: | hybrid plasmid pBLTR- luc was constructed,which contains firefly luciferase gene under the control of BIV LTR promoter. Luciferase activity could be detected while EBL-1cells were transfected with pBLTR-luc. Plasmid pBIV tat could significantly transactivate theexpression of luciferase gene,which implied that LTR could work normally . Luciferase activity could obviously be detected when EBL- 1 cells were transfected with plasmid DNA as less as10ng.Compared with the LTR-CAT assay system, LTR-luc assay system is more rapid,simpler and more sensitive. |
| |
| Keywords: | Firefly luciferase gene(luc ) BIV-LTR BIV-tatInstitute of Microbiology |
| 本文献已被 CNKI 等数据库收录！ |
