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Hyaluronic acid synthesis in a cell-free system from rat fibrosarcoma |
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| Authors: | J J Hopwood F W Fitch A Dorfman J P Kennedy |
| Affiliation: | 4. Departments of Pediatrics and Biochemistry, Joseph P. Kennedy, Jr., Mental Retardation Research Center, University of Chicago, Chicago, Illinois 60637 USA;1. Department of Pathology, Pritzker School of Medicine, University of Chicago, Chicago, Illinois 60637 USA |
| Abstract: | A transplantable rat fibrosarcoma (in both ascites and solid forms) has been shown to contain and synthesize large amounts of hyaluronic acid. A particulate hyaluronic acid synthesizing system has been isolated from the solid fibrosarcoma and some characteristics of the enzyme system are detailed. The enzyme complex transferred GlcUA or GlcNAc from UDP-GlcUA or UDP-GlcNAc at a rate of approximately 60 nmole/hr/mg protein to extend hyaluronic acid chains by approximately 45,000 daltons. |
| Keywords: | diHA ΔdiHA SDS sodium dodecyl sulfate |
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