Antigenic Reactivity of a Monoclonal Antibody Against Amoeba proteus Actin |
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Authors: | TAE I AHN KWANG W JEON |
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Institution: | Department of Zoology, University of Tennessee, Knoxville, Tennessee 37996 |
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Abstract: | The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii , but it did not react with Naegleria gruberi actin. The amoeba actin was resolved into 3 bands with isoeletric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2-dimensional immunoblots. |
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Keywords: | Actin Amoeba monoclonal antibody |
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