Different Functional and Structural Properties of Lactate Dehydrogenase Isozymes at Different Stages of Danio rerioOntogenesis |
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Authors: | Klyachko O S Ozernyuk N D |
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Institution: | (1) Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991, Russia |
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Abstract: | We studied properties of lactate dehydrogenase isozymes expressed at different stages of Danio rerioontogenesis. H4-LDH and a minor fraction H3M1are expressed during embryonic development. The muscle isozyme ( 4) appears after the beginning of muscle contractions in the embryo. 4and 4isozymes isolated from the heart and skeletal muscle of the adult fish, respectively, show significant differences in terms of Michaelis constant (K
m) activation energy (AE), and inactivation temperature. H4-LDH isozymes isolated from unfertilized eggs, the skeletal muscle of larvae, and the heart of the adult fish differ inK
mand activation energy, as well as in inactivation temperature. We propose that these differences may be associated with a ligand interacting with the H4isozyme at different steps of ontogenesis. |
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Keywords: | early ontogenesis lactate dehydrogenase H4-LDH and M4-LDH isozymes Michaelis constant (K
m) activation energy (E
a) Danio rerio |
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