Oil droplets and yolk spheres during development of Medaka embryos |
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Authors: | Takashi Iwamatsu Tomokazu Muramatsu Hirokuni Kobayashi |
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Institution: | (1) Department of Biology, Aichi University of Education, Igaya, Kariya, Aichi 448-8542, Japan;(2) Department of Biology, Asahi University School of Dentistry, 1851 Hozumi, Mizuho, Gifu 501-0296, Japan |
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Abstract: | The sizes of oil droplets (globules) and the yolk sphere in the Medaka Oryzias latipes egg were measured in the developmental period from fertilization to hatching. Oil droplets coalesced with one another in
the process of shifting toward the vegetal pole, and a single large oil droplet was finally located at the vegetal pole region
in most eggs 2 days post-fertilization. The volume of the yolk sphere steeply decreased in the period from 2 to 8 days post-fertilization.
The volume of oil droplets also declined linearly from 4 to 10 days post-fertilization. Lipid components exhibited no distinct
change during embryogenesis. In order to verify whether oil droplets were required for development of Medaka embryos, oil
droplets were artificially removed from the early developing embryos without the chorion (egg envelope). Naked embryos without
the oil droplet developed normally to fry in the sterilized incubation medium and grew to the same mature fry as those grown
from the control embryos. |
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Keywords: | Oil droplet Yolk sphere Embryonic development Oryzias latipes Dechorionation |
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