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Development of new VNTR markers for pike and assessment of variability at di- and tetranucleotide repeat microsatellite loci
Authors:M M Hansen    J B Taggart  † D Meldrup
Institution:Danish Institute for Fisheries Research, Department of Inland Fisheries, Vejlsøvej 39, DK-8600 Silkeborg, Denmark;Institute of Aquaculture, University of Stirling, Stirling FK9 4LA, Scotland, U.K.
Abstract:Levels of variation at six VNTR (variable number of tandem repeats) loci, one minisatellite and five microsatellite loci, isolated from tri- and tetranucleotide enriched DNA libraries for northern pike were generally low in two Danish populations (1–4 alleles; expected heterozygosity 0–0·57), though one highly variable microsatellite (13 alleles; expected heterozygosity 0·79) was identified. In combination with previously published microsatellites a set consisting of nine polymorphic loci appeared to be useful for discriminating populations, as determined by assignment tests.
Keywords:Esox lucius            microsatellite  minisatellite  enriched DNA library  genetic variation
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