Screening for a novel enzyme hydrolysing l-carnitine amide |
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Authors: | Ulrich Joeres Maria Regina Kula |
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Institution: | (1) Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, D-52404 Jülich, Postfach, 2050, Germany |
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Abstract: | In an extended screening using d,l-carnitine amide as carbon or nitrogen source about 1300 strains were obtained by enrichment culture. Of these, 65 strains possessed carnitine amidase activities. A single strain was identified as containing an enzyme able to hydrolyse only l-carnitine amide and yield carnitine of high enantiomeric purity ( 97) when incubated with the racemic substrate. During the initial optimisation of the culture conditions the volume activity could be improved 6.7-fold whereas the specific activity increased 3.6-fold. The enzyme is inducible by l-carnitine amide and carnitine and to a lesser degree also by -butyrobetaine and dehydrocarnitine. As judged by the fatty acids and quinone composition the strain belongs into the -subgroup of purple bacteria but has not yet been classified by the German Culture Collection into a known genus of bacteria.
Correspondence to: M.-R. Kula |
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