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Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor |
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| Authors: | Hyou-Arm Joung Won-Bo Shim Duck-Hwa Chung Junhyoung Ahn Bong Hyun Chung Ho-Suk Choi Sang-Do Ha Keun-Sung Kim Kyu-Ho Lee Cheol-Ho Kim Kwang-Yup Kim Min-Gon Kim |
| Institution: | 1. BioNanotechnology Research Center, KRIBB, 305-806, Daejeon, Korea 2. Department of Chemical Engineering, Chungnam National University, 305-701, Daejeon, Korea 3. Department of Food Science and Technology, Gyeongsang National University, 660-701, Jinju, Korea 4. Department of Food Science and Technology, Chung-Ang University, 155-756, Seoul, Korea 5. Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies, 449-791, Yongin, Korea 6. Department of Biological Sciences, College of Natural Science, Sungkyunkwan University, 440-746, Suwon, Korea 7. Department of Food Science and Technology, Chungbuk National University, 361-763, Cheongju, Korea |
| Abstract: | In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method and utilized repeatedly by regeneration. The monoclonal antibody, ‘A18’, was selected and employed for the high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 103 cells/ml or 50 cells were detected by the SPR biosensor. |
| Keywords: | surface plasmon resonance (SPR) protein L Listeria monocytogenes antibody |
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