Eltor型霍乱弧菌L型的诱导及形态学观察

本文主要介绍Eltor型霍乱弧菌在液体和固相的青霉素诱导下形成L型的过程及其菌落和菌体的形态学改变。Eltor型霍乱弧菌在被诱导3—5次即可出现典型的L型菌落。本试验共诱导10次,获得稳定的L型菌株,菌落形态除油煎蛋样外,还可见到双重心样菌落、靶环样菌落、纤维型菌落。菌体的正常状态为徽弧状,经青霉素作用后,微弧状消失,膨大变直,继尔变成杆状和杆状长链。菌落中心多为相互盘绕、交织成网状的丝状体。

INDUCEMENT AND MORPHOLOGIC OBSERVATION OF L-FORM OF VIBRIO CHOLERAE, EL TOR BIOTYPE

L-forms of Vibrio cholerae, El Tor biotype were induced by penicillin under the condition of liquid and solid media, and observed morphologically. It showed that the typical L-form colonies like oil-fried egg (L-form), granular form (Gform) and fibrous form (F-form) were seen after 3-10-time inducements under the microscope. At the beginning, it was observed that the bodies of bacteria expanded, and then became short-rod, long-chain and finally fibre-like bodies.