Modulation of phospholipase D stimulation in c-src transfected mesangial cells |
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| Institution: | 1. Department of Emergency, The Dingli Clinical College of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China;2. Department of Pharmacy, the Second Affiliated Hospital & Yuying Children''s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China;1. Department of Pathology, The Second Affiliated Hospital and Yuying Children’s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China;2. Department of Anesthesiology and Perioperative Medicine, The Second Affiliated Hospital and Yuying Children’s Hospital, Key Laboratory of Pediatric Anesthesiology, Ministry of Education, Key Laboratory of Anesthesiology of Zhejiang Province, Wenzhou Medical University, China |
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| Abstract: | Stimulation of rat mesangial cells with platelet-derived growth factor BB (PDGF-BB) enhanced phospholipase D (PLD) activity in a concentration dependent manner. Mesangial cells overexpressing the tyrosine kinase pp60c-src (c-Src) were used to determine the effect of this non transforming protooncogene on PLD activation. Overexpression of c-Src interfered with PDGF-BB-mediated activation of PLD. This modulation was dependent on the tyrosine kinase activity of c-Src, since overexpression of tyrosine kinase-negative mutants of c-Src did not affect PLD activation. No effect of c-Src overexpression was observed, when PLD was activated by ATP or guanosine 5′-3-O-(thio)triphosphate (GTPγS). The results indicate that the tyrosine kinase c-Src specifically interfered with PDGF-mediated but not with ATP- or GTPγS-mediated PLD activation. |
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