A simple assay for fluorescent siderophores produced by Pseudomonas species and an efficient isolation of pseudobactin |
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Authors: | Brian Nowak-Thompson Steven J Gould |
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Institution: | (1) Department of Chemistry, Oregon State University, Corvallis, OR, USA;(2) Department of Chemistry, Oregon State University, 97331-4003 Corvallis, OR, USA |
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Abstract: | Several iron binding metabolites (siderophores) of Pseudomonas fluorescens B10 (JL-3133) have been detected using C18 reverse phase HPLC coupled with photodiode array detection methods. This analysis utilized a volatile mobile phase of 90% 20 mm NH4HCO3/10% MeOH, pH 6.5. It has been shown to be applicable to other P. fluorescens strains for the detection of related metabolites. Direct scale-up of the analytical HPLC conditions allowed for the efficient preparative isolation of pseudobactin, the principle siderophore produced by P. fluorescens B10 (JL-3133). |
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Keywords: | HPLC pseudobactin Pseudomonas fluorescens siderophores |
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