Comparison of the immunogenicities of HIV-1 mutants based on structural modification of env |
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Authors: | Jian-hui Nie Chun-tao Zhang Hui-hui Chong Xue-ling Wu Chun-yu Liu Yu Wu Chen-yan Zhao Lin-qi Zhang You-chun Wang |
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Institution: | (1) Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, No. 2 Tiantanxili, Beijing, 100050, China;(2) College of Life Science, Jilin University, Changchun, 130012, China;(3) Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Avenue, New York, NY 10016, USA |
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Abstract: | Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable
regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two
pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities
for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was
only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral
immune responses.
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Keywords: | HIV-1 Env Modification Neutralization assay ELISPOT |
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