Freezing induces biased results in the molecular detection of Flavobacterium columnare |
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| Authors: | Suomalainen Lotta-Riina Reunanen Hilkka Ijäs Ritva Valtonen E Tellervo Tiirola Marja |
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| Affiliation: | Department of Biological and Environmental Science, University of Jyv?skyl?, P.O. Box 35, FIN-40014 University of Jyv?skyl?, Finland. |
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| Abstract: | Specific PCR detection and electron microscopy of Flavobacterium columnare revealed the risk of false-negative results in molecular detection of this fish pathogen. Freezing and thawing destroyed the cells so that DNA was for the most part undetectable by PCR. The detection of bacteria was also weakened after prolonged enrichment cultivation of samples from infected fish. |
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