a Faculty of Chemical Engineering of Lorena, Biotechnology Department, C.P.116. 126000-000, Lorena, SP, Brazil
b Cuban Institute for Research on Sugarcane Derivatives, P.O. Box 4026, Havana, Cuba
Abstract:
Continuous fermentation of sugarcane bagasse hemicellulosic hydrolysate by the yeast Candida guilliermondii FTI 20037 was used for xylitol production from xylose. Experiments were carried out in a reactor with 1.25 l of treated hydrolysate, at 30 °C and 300 rpm. A 22 full-factorial central composite design was employed for experimental study and analysis of the results. A statistical analysis of the results showed that the effects of the pH and dilution rate (D), the interactions between these variables and the second-order effect of D on the xylitol volumetric productivity (Qp) were significant at a 95% confidence level. The second-order effect of pH was also significant at a 90% confidence level. The kLa effect on the Qp was not significant. A volumetric productivity of 0.68 g/l h, representing 95.8% of the predicted value (0.72 g/l h), was obtained.