Two enzyme active transport in vitro with ph induced asymmetrical functional structures: II. Properties of the pump and experimental illustrations

The theory and practice of the pump are illustrated. With graphs drawn using the analytical equations developed in Part I. the optimizing effect of reactive and diffusive parameters of the membrane or of pH gradients are discussed as a function of boundary conditions. Profile symmetries, extractive and accumulating powers, time constants and stereoselectivities are examined. An experimental glucose pump demonstrates the feasibility of the theoretical predictions. A mixture of hexokinase (optimal pH = 8.2) and phosphatase (optimal pH = 6.7) was immobilized in an agarose membrane. When asymmetry was induced by a pH gradient (between 6 and 9), ATP was consumed, D-glucose accumulated on one side and was separated from the L-isomer. Further generalizations and extensions are commented on.