Isoenzymes of ferredoxin-NADBΔ oxidoreductase from the cyanobacterium Nostoc strain MAC |
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| Authors: | Georgina N Hutber Lyndon J Rogers |
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| Institution: | (1) Department of Biochemistry, University College of Wales, SY23 3DD Aberystwyth, Dyfed, Wales, UK;(2) Present address: Department of Biochemistry and Soil Science, University College of North Wales, LL57 2UW Bangor, Gwynedd, Wales, UK |
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| Abstract: | Ferredoxin-NADP+ oxidoreductase from the cyanobacterium Nostoc strain MAC was separated into two fractions by ion-exchange chromatography. Both were purified to electrophoretic homogeneity and exhibited diaphorase and ferredoxin-dependent cytochrome c reductase activity. The activities with three different electron carriers in this latter assay were similar for the two fractions, as were the pH optima in both assays. Each fraction, however, could be resolved into several active components by isoelectric focusing, both after initial separation and following apparent purification by gel filtration on Sephadex G-150, further chromatography on DEAE-cellulose, and use of hydroxylapatite columns.Abbreviation DCIP =
phenolindo-3,6-dichlorophenol> |
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| Keywords: | cyanobacterium ferredoxin-NADP+ oxidoreductase isoelectric focusing isoenzymes |
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