合成气发酵制取乙醇微生物的对比

为了验证富集菌群利用合成气产乙醇的能力,以富集培养获得的4种菌群A-fm4、G-fm4、LP-fm4、B-fm4为研究对象,在10％接种量和25％接种量两种情况下,与合成气发酵菌株梭状芽胞杆菌Clostridiumautoethanogenum进行对比.结果表明:10％接种量时,A-fm4、G-fm4、LP-fm4、B-fm4与C.autoethanogenum乙醇产量分别为349.15、232.16、104.25、79.90和26.99mg/L；25％接种量发酵时,乙醇产量分别为485.81、472.73、348.58、272.52和242.15 mg/L.提高接种量能增加乙醇的产量.富集菌群乙醇产量与目前报道的C.autoethanogenum最高产量(259.64 mg/L)相比具有显著优势,提供了一种提高合成气发酵生产乙醇产量的方法.

Comparison of microorganisms fermenting syngas into ethanol

To evaluate the ability of microbial mix-culture fermenting syngas into ethanol, we studied the microbial mix-cultures A-fm 4, G-fm 4, Lp-fm 4 and B-fm 4 obtained by enrichment and compared with Clostridium autoethanogenum DSM10061 with 10% and 25% inoculation size. The results show that, with 10% inoculation size, the ethanol production of A-fm 4, G-fm 4, Lp-fm 4, B-fm 4 and C. autoethanogenum were 349.15, 232.16, 104.25, 79.90 and 26.99 mg/L respectively. With 25% inoculation size, the ethanol production were 485.81, 472.73, 348.58, 272.52 and 242.15 mg/L respectively. Higher inoculation size will increase the production of ethanol. The tested mix-culture exhibited a significant yield advantage compared with the maximum production of C. autoethanogenum reported in the literature (259.64 mg/L). This research provided a practical method to improve ethanol production from syngas.