High cell density and high expression of recombinant human ApoA-IMilano in Escherichia coli by twice temperature-shifted induction |
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| Authors: | Yingping Zhuang Wenfeng Ma Meijin Guo Mansheng Ding Ju Chu Siliang Zhang |
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| Affiliation: | 1. State Key Laboratory of Bioreactor Engineering, National Research Center for Biotechnology, East China University of Science and Technology, Shanghai, 200237, China
2. State Key Laboratory of Bioreactor Engineering and National Research Center for Biotechnology, East China University of Science and Technology, Shanghai, 200237, China
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| Abstract: | The effect of temperature on the formation of recombinant protein, apolipoprotein A-IMilano was investigated in the present study. The temperature of the initial growth phase was set at 30°C, while temperature variation in induction phase was arranged in three modes. High cell-density culture of Escherichia coli and high expression of recombinant human by twice temperature-shifted induction were carried out. Experimental results showed that ApoA-IMilano reached 4.8 g/L with the final cell density of OD600, 150. It was found that twice temperature-shifted induction could successfully avoid the effect of acetic acid on cell density and the expression of the product. The present study provides a basic procedure for the production of recombinant ApoA-IMilano. |
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