|
|||||
|
|
Blue and yellow laccases of ligninolytic fungi |
|
| Authors: | Alexei A Leontievsky Tamara Vares Pauliina Lankinen Jasvinder K Shergill Natalia N Pozdnyakova Nina M Myasoedova Nisse Kalkkinen Ludmila A Golovleva Richard Cammack Christopher F Thurston Annele Hatakka |
| Institution: | Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia;Department of Applied Chemistry and Microbiology, P.O. Box 56 (Biocenter 1), University of Helsinki, FIN-00014 Helsinki, Finland;Institute of Biotechnology, P.O. Box 56 (Biocenter 1), University of Helsinki, FIN-00014 Helsinki, Finland;Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK |
| Abstract: | Extracellular laccases from submerged cultures of Coriolus versicolor BKM F-116, Panus tigrinus 8/18, Phlebia radiata 79 (ATCC 64658), Phlebia tremellosa 77-51 and from cultures of Pa. tigrinus 8/18, Ph. radiata 79 and Agaricus bisporus D-649 grown on wheat straw (solid-state fermentation) were purified. All enzymes from submerged cultures had a blue colour and characteristic absorption and EPR spectra. Laccases from the solid-state cultures were yellow-brown and had no typical blue oxidase spectra and also showed atypical EPR spectra. Comparison of N-terminal amino acid sequences of purified laccases showed high homology between blue and yellow-brown laccase forms. Formation of yellow laccases as a result of binding of lignin-derived molecules by enzyme protein is proposed. |
| Keywords: | Ligninolytic fungus Blue copper oxidase Laccase Absorption EPR spectrum |
| 本文献已被 ScienceDirect 等数据库收录！ | |