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Best practices in cell culture: an overview
Authors:John M Baust  Gertrude Case Buehring  Lia Campbell  Eugene Elmore  John W Harbell  Raymond W Nims  Paul Price  Yvonne A Reid  Frank Simione
Institution:1.CPSI Biotech,Owego,USA;2.Gertrude Case Buehring UC Berkeley, School of Public Health,Berkeley,USA;3.Tissue Testing Technologies, LLC,North Charleston,USA;4.Department of Radiation Oncology, UC,Irvine,USA;5.JHarbell Consulting, LLC,Dallas,USA;6.RMC Pharmaceutical Solutions, Inc.,Longmont,USA;7.Mount Pleasant,USA;8.ATCC,Manassas,USA;9.ATCC,Manassas,USA
Abstract:This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells.
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