Physiological and biochemical features of embryogenic and non-embryogenic peach palm (<Emphasis Type="Italic">Bactris gasipaes</Emphasis> Kunth) cultures |
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Authors: | Maria Carolina Andrade Nascimento-Gavioli Gabriela Claudia Cangahuala-Inocente Douglas Steinmacher Joseph Francis Ree Neusa Steiner Miguel Pedro Guerra |
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Institution: | 1.Laboratory of Plant Developmental Physiology, Graduate Program in Plant Genetic Resources,Federal University of Santa Catarina,Florianópolis,Brazil;2.Vivetech Agrosciences,Marechal Candido Rondon,Brazil |
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Abstract: | Both embryogenic and non-embryogenic peach palm (Bactris gasipaes Kunth) cultures arise during somatic embryogenesis induction, and both tissue types are often observed growing side-by-side from the same explant. To better understand why this occurs, samples from each tissue type were analyzed for their endogenous concentrations of indole-3-acetic acid (IAA), abscisic acid (ABA), polyamines, and amino acids with high-performance liquid chromatography and for total phenolics with spectrophotometry. Embryogenic cultures contained significantly higher concentrations of IAA, ABA, and total amino acids, whereas non-embryogenic tissue contained more total polyamines and phenolics. The greater IAA concentrations in embryogenic cultures supported the role of that hormone as a marker of embryogenic potential. Putrescine was especially prevalent in non-embryogenic cultures; however, the decreased putrescine/spermine + spermidine ratio in embryogenic cultures added support to the conclusions of previous studies in other species that this can serve as a marker of embryogenic competence. Though embryogenic cultures contained higher total amino acids, each culture type had different concentrations of specific amino acids. |
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