首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Genetic and biochemical stability assessment of plants regenerated from cryopreserved shoot tips of a commercially valuable medicinal herb <Emphasis Type="Italic">Bacopa monnieri</Emphasis> (L.) Wettst
Authors:Neelam Sharma  Rakesh Singh  Ruchira Pandey  Nutan Kaushik
Institution:1.Tissue Culture and Cryopreservation Unit,ICAR-National Bureau of Plant Genetic Resources,New Delhi,India;2.Division of Genomic Resources,ICAR-National Bureau of Plant Genetic Resources,New Delhi,India;3.Tata Energy Research Institute,New Delhi,India
Abstract:Shoot tips from four accessions (IC249250, IC 426442, IC 375976, and IC468878) of Bacopa monnieri (L.) Wettst., a commercially valuable memory revitalizing medicinal plant, were cryopreserved using a vitrification technique. Depending on the genotype, 0 to 20% plant regeneration without intermediary callus was achieved from cryopreserved shoot tips. Genetic stability of plants derived from cryopreserved shoot tips was assessed using biochemical and molecular markers. The regenerated plants from non-frozen controls and cryopreserved shoot tips exhibited morphological similarity to respective parental material when transferred to soil. On the basis of ten random amplified polymorphic DNA (RAPD) and bacoside A content using HPLC analysis, no significant reproducible variation was observed between the controls and in vitro-cryopreserved plants. Thus, after cryopreservation treatment, the regenerated plants exhibited molecular and biochemical genetic stability.
Keywords:
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号