Construction and analysis of miniplasmids of the colicin Ib plasmid |
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Authors: | Satish K. Walia Donna H. Duckworth |
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Affiliation: | Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville, Florida 32610 U.S.A. |
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Abstract: | Miniplasmids of the colicin Ib (ColIb) plasmid have been isolated from two Tn5-induced mutants of ColIb and their structure determined. These have then been used to order the sequence of restriction endonuclease fragments of the whole plasmid. In addition, the sites of the colicin, colicin immunity, and abortive infection gene have been determined in relation to the restriction sites. By comparison of the miniplasmids with other “I” incompatibility group plsmids, the probable location of the incompatibility gene and the origin of replication have been confirmed. |
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